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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
Fecha : |
29/09/2014 |
Actualizado : |
25/11/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
GIL, J.; GENOVESE, P.; SARTORE, I.; BIELLI, A.; MONTOSSI, F.; VIÑOLES, C. |
Afiliación : |
J. GIL, Universidad de la República (UdelaR)/ Facultad de Veterinaria; P. GENOVESE, Universidad de la República (UdelaR)/ Facultad de Veterinaria; I. SARTORE, Universidad de la República (UdelaR)/ Facultad de Veterinaria; A. BIELLI, Universidad de la República (UdelaR)/ Facultad de Veterinaria; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA VIÑOLES GIL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
The leptin receptor is expressed in the Leydig cells of Merino rams, but its expression is not affected by long-term differences in the plane of nutrition |
Fecha de publicación : |
2014 |
Fuente / Imprenta : |
Animal Production Science, 2014 v. 54, p. 981-986. |
DOI : |
10.1071/AN12320 |
Idioma : |
Inglés |
Notas : |
Article history: Received 12 September 2012 / Accepted 26 September 2013 / Published online 19 December 2013. |
Contenido : |
ABSTRACT.
We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement of nutrition promoted histological changes of increased spermatogenesis of Merino rams, but was not related to changes in the expression of the Ob-Rb receptor in the Leydig cell cytoplasm. MenosABSTRACT.
We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement... Presentar Todo |
Thesagro : |
MERINO; NUTRICIÓN ANIMAL; OVINOS. |
Asunto categoría : |
-- L51 Fisiología Animal - Nutrición |
Marc : |
LEADER 02510naa a2200241 a 4500 001 1052570 005 2019-11-25 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1071/AN12320$2DOI 100 1 $aGIL, J. 245 $aThe leptin receptor is expressed in the Leydig cells of Merino rams, but its expression is not affected by long-term differences in the plane of nutrition 260 $c2014 500 $aArticle history: Received 12 September 2012 / Accepted 26 September 2013 / Published online 19 December 2013. 520 $aABSTRACT. We studied whether the long-term effects of nutrition on ram reproductive potential are mediated by changes in the expression and distribution of leptin receptor (Ob-Rb) in the testes. Thirty-two young Merino rams were allocated to a 2 × 2 factorial design, with either natural (NP) or improved pastures (IP), with (+S) or without (?S) supplementation. After 14 weeks of treatment, a blood sample was taken to measure leptin concentrations and rams were castrated. The weights of testicles (TW) and epididymides (EW) were recorded before tissue sampling for the detection of Ob-Rb expression by immunohistochemistry. TW was positively affected by the interaction IP × S, but not EW and bodyweight (BW), which were affected either by IP or S (P < 0.05). TW and EW were greatest (g) in IP+S rams (290.6 ± 40.1 and 57.6 ± 2.3), followed by IP?S (284.6 ± 61.4 and 52.5 ± 2.3), NP+S (282.6 ± 37.9 and 55.8 ± 2.3), and NP?S rams (208.5 ± 48.7 and 44.4 ± 2.3; P < 0.01). Expression of Ob-Rb was observed in the cytoplasm of Leydig cells, without any effect of treatments on the percentage of positive Leydig cells or on staining-intensity score. The serum leptin concentrations were similar among groups. The absolute volume of the seminiferous epithelium (mL) was affected by IP and S, as well as by their interaction (IP?S: 179.7 ± 13.1, IP+S; 174.9 ± 9.0; NP+S: 178.0 ± 10.2; NP?S: 120.8 ± 9.8; P < 0.05). We conclude that, under the conditions of the present study, the long-term improvement of nutrition promoted histological changes of increased spermatogenesis of Merino rams, but was not related to changes in the expression of the Ob-Rb receptor in the Leydig cell cytoplasm. 650 $aMERINO 650 $aNUTRICIÓN ANIMAL 650 $aOVINOS 700 1 $aGENOVESE, P. 700 1 $aSARTORE, I. 700 1 $aBIELLI, A. 700 1 $aMONTOSSI, F. 700 1 $aVIÑOLES, C. 773 $tAnimal Production Science, 2014$gv. 54, p. 981-986.
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INIA Las Brujas (LB) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
09/03/2017 |
Actualizado : |
30/09/2019 |
Tipo de producción científica : |
Poster |
Autor : |
SCHILD, C.; GIANNITTI, F.; MEDEIROS,R.; SILVEIRA, CS.; CAFFARENA, D.; POPPENGA, RH.; RIET-CORREA, F. |
Afiliación : |
CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Acute lead arsenate poisoning in beef cattle in Uruguay. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
In: American Association of Veterinary Laboratory Diagnosticians (AAVLD), 59º; United States Animal Health Association (USAHA) Annual Meeting, Greensboro, North Carolina, Oct 13-19, 2016. |
DOI : |
10.1177/1040638719831413 |
Idioma : |
Inglés |
Palabras claves : |
ARSENIATO DE PLOMO. |
Thesagro : |
BOVINOS; ENVENENAMIENTO; URUGUAY. |
Asunto categoría : |
-- |
Marc : |
LEADER 00790nam a2200229 a 4500 001 1056812 005 2019-09-30 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1177/1040638719831413$2DOI 100 1 $aSCHILD, C. 245 $aAcute lead arsenate poisoning in beef cattle in Uruguay.$h[electronic resource] 260 $aIn: American Association of Veterinary Laboratory Diagnosticians (AAVLD), 59º; United States Animal Health Association (USAHA) Annual Meeting, Greensboro, North Carolina, Oct 13-19$c2016 650 $aBOVINOS 650 $aENVENENAMIENTO 650 $aURUGUAY 653 $aARSENIATO DE PLOMO 700 1 $aGIANNITTI, F. 700 1 $aMEDEIROS,R. 700 1 $aSILVEIRA, CS. 700 1 $aCAFFARENA, D. 700 1 $aPOPPENGA, RH. 700 1 $aRIET-CORREA, F.
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